Molecular Approach for Identification of Pangasianodon Hypophthalmus based on Mitochondrial Cytochrome b Oxidase subunit i (coi) Gene from Pakistan

The aquaculture industry is dependent on rich fish resources in water bodies. Human activities have led to a rapid decline of species. In Asia, the Pangasiidae family highly valued for its potential survival and fillet meat. DNA barcoding taxonomic method using genetic markers organisms mitochondrial (mt DNA) identification. phylogeny identification Pangasianodon hypophthalmus subcontinent great concern. For species identification, precise technique barcoding. This strongly effective analyzing divergence among more reliable as compared external morphology. To avoid mislabeling conservation species, it equally useful juveniles well adult stages fishes. As bar-coding that uses small organisms’ particular recent study MAGA X Kimura 2 Parameter was used evaluate distance neighbor joining tree constructed. BOLD GenBank reveals nearest identity matches. cyt-b gene region successfully identifying accepted standard